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Applications Key
WB = Western Blot
IP = Immunoprecipitation
IAC= Immunoaffinity Column
ChIP= Chromatin Immunoprecipitation
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RNA Pol II Antibodies arrow RNA Pol II RPB1 Monoclonal Antibody
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RNA Pol II RPB1 Monoclonal Antibody
RNA Pol II RPB1 Monoclonal Antibody
Clone: 8WG16 Product Format: Affinity Purified
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Catalog #: WP011 - $275.00  
Size: 100 µg

Details:
Tested Applications
WB, ELISA
Target Information
RPB1 is a 220kDa sub-unit of RNA Polymerase II
Product Format
Affinity Purified
Alternate Names
RNA polymerase II sub-unit 1, RPB1, CTD repeat YSPTSPS, C- terminal domain repeat YSPTSPS, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, POLR2A, POLR2
Clone
8WG16
Host
Mouse
Isotype
IgG2a
Immunogen
Purified protein
Epitope
YSPTSPS repeat
Relevance
RPB1 is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
Specificity
RNA Pol II RPB1 human and yeast
Reported Reactivity
wheat, mouse, C. elegans, X. laevis
Reported Applications
ChIP, Transcription inhibition, IP

Technical Data Sheet Technical Data Sheet

 

Relevant Links:

NCBI NP_000928

Swiss Prot P24928

Literature Search

 

References:

1. Edward, A.M., Darst, S.A., Feaver, W.J., Thompson, N.E., Burgess, R.R., and Kornberg, R.D.(1990). Purification and lipid-layer crystallization of yeast RNA polymerase II. Proc. Natl. Acad. Sci. USA 87, 2122-6.

2. Thompson, N.E., Aronson, D.B., and Burgess, R.R. (1990). Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from polyol-responsive monoclonal antibody. J Biol Chem 265, 7069-77.

3. Thompson, N.E., and Burgess, R.R. (1989). Applications of monoclonal antibodies to the study of eukaryotic RNA polymerase. IN :Molecular Biology of Chromosome Function (Adolf, K., W., ed), pp 180-206, Springer Verlag, New York, NY.

4. Thompson, N.E.,Steinberg, T. H., Aronson, D.B., and Burgess, R.R. (1989). Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J. Biol. Chem. 264, 11511-20.

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