Neoclone - We excel in the manufacture of monoclonal antibodies
Search

Can't Find It? Try Our Site Map
Product Catalog Custom Services Company Support
 

SUPPORT
References

Catalog References
Polyol-Responsive mAbs and Immunochromatography References
ABL-MYC References
References Citing NeoClone Antibodies
Epitope Tag Immunopurification

Catalog References

  1. Breyer, M.J., Thompson, N.E., and Burgess, R.R.,(1997). Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase. J. Bacteriol. 179, 1404-8.

  2. Erickson, B.D. (1990). NUSA, the RPSU-DNAG-RPOD operon, and the metabolism of RNA in Escherichia coli. Ph.D. dissertation, University of Wisconsin-Madison, pp. 223.

  3. Jovanovich, S. B., Lesley, S. A., and Burgess R. R. (1989) In vitro use of monoclonal antibodies in E. coli S-30 extracts to determine the RNA polymerase sigma subunit required by a promoter. J. Biol. Chem. 264, 3794-98.

  4. Lesley, S.A., (1989). An immunological approach to studying transcription initiation in E.coli. Ph.D. dissertation, University of Wisconsin-Madison, pp. 201.

  5. Lesley, S.A., and Burgess R. R. (1989). Characterization of Escherichia coli transcription factor sigma 70: localization of a region involved in the interaction with core RNA polymerase. Biochemistry 28,7728-34.

  6. Lesley, S.A., Thompson, N.E., and Burgess R. R. (1987). Studies of the role of the E.coli heat shock regulatory protein sigma 32 by the use of monoclonal antibodies . J. Biol. Chem. 262, 5404-7.
  7. Nguyen, L.H., Jensen, D.B., Thompson, N. E., Gentry, D. R., and Burgess R. R. (1993). In vitro functional characterization of overproduced E. coli katF/rpoS gene product. Biochemistry 32, 11112-7.
  8. Strickland, M. S., Thompson, N. E., and Burgess R. R.(1988). Structure and function of the sigma70 subunit of Escherichia coli RNA polymerase. Monoclonal antibodies: localization of epitopes by peptide mapping and effects and transcription, Biochemistry 27, 5755-62.

  9. Thompson, N. E., Hager, D. A., and Burgess R. R.(1992). Isolation and characterization of polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase. Biochemistry 31, 7003-8.

  10. Edward, A.M., Darst, S.A., Feaver, W.J., Thompson, N.E., Burgess, R.R., and Kornberg, R.D.(1990). Purification and lipid-layer crystallization of yeast RNA polymerase II. Proc. Natl. Acad. Sci. USA 87, 2122-6.

  11. Svetlov,V., Nolan, K., and Burgess, R.R. (1998). Rpb3, stoichiometry and sequence determinants of the assembly into yeast RNA polymerase II in vivo. J Biol Chem 273, 10827-30.

  12. Thompson, N.E., Aronson, D.B., and Burgess, R.R. (1990). Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from polyol-responsive monoclonal antibody. J Biol Chem 265, 7069-77.

  13. Thompson, N.E., and Burgess, R.R. (1989). Applications of monoclonal antibodies to the study of eukaryotic RNA polymerase. IN :Molecular Biology of Chromosome Function (Adolf, K., W., ed), pp 180-206, Springer Verlag, New York, NY.

  14. Thompson, N.E.,Steinberg, T. H., Aronson, D.B., and Burgess, R.R. (1989). Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J. Biol. Chem. 264, 11511-20.

  15. Thompson, N.E., and Burgess, R.R. (1994). Purification of recombinant human transcription factor IIB by immunoaffinity chromatography. Protein Expression Purif. 5, 468-75.

  16. Thompson, N.E., and Burgess, R.R. (1999) Immunopurification of the RAP30 subunit of human transcription factor IIF (TFIIF). Protein Expression Purif., 17, 260-266.

  17. Thompson, N.E., Strasheim, L.A., Nolan, K.M., and Burgess, R.R. (1995). Accessibility of epitopes on human transcription factor IIB in the native protein and in a complex with DNA. J. Biol. Chem. 270, 4735-40.
  18. Anthony, L.C. Foley, K. Thompson, N. and Burgess R. R. (2003) Expression, purification of, and monoclonal antibodies to the sigma factors from E. coli. Methods in Enzymology, 370, 181-192.
  19. Bergendahl, V., Thompson, N., Foley, K., Olson, B., and Burgess, R.R. (2003). A cross-reactive polyol-responsive monoclonal antibody useful for isolation of core RNA polymerase from many bacterial species. Protein Expression and Purification, 31, 155-160.
  20. Erickson, B. University of Wisconsin-Madison PhD Thesis. Chapter 3: Isolation and Characterization of Monoclonal Antibodies to NusA, pp50-82, 1990.
  21. Thompson, N., Arthur T., and Burgess, R.R. (2003). Development of a new epitope tag for the gentle purification of proteins by immunoaffinity chromatography. Anal. Biochem. 323,171-179.
  22. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W., and Prasher, D.C. (1994) Green Fluorescent Protein as a Marker for Gene Expression. Science 263, 802-805.
  23. Thompson, N.E., Foley, K.M., and Burgess, R.R. (2004) Antigen-binding properties of monoclonal antibodies reactive with human TATA-binding protein and use in immunoaffinity chromatography.
    Protein Expression and Purification 36, 186-97.
  24. Grainger, D. C., Hurd, D., Harrison, M., Holdstock, J. and Busby, S.J. W. (2005) Studies of the distribution of Escherichia coli cAMP-receptor protein and RNA polymerase along the E. coli chromosome.
    Proceeding of the National Academy of Science 102, 17693-17698.
  25. Koehler JE, Burgess RR, Thompson NE, Stephens RS. (1990) Chlamydia trachomatis RNA polymerase major sigma subunit. Sequence and structural comparison of conserved and unique regions with Escherichia coli sigma 70 and Bacillus subtilis sigma 43. J Biol Chem. 265(22):13206-14. Erratum in: J Biol Chem 1990 Dec 5;265(34):21390.

Polyol-Responsive mAbs and Immunochromatography References

  1. Thompson, N. E., Steinberg, T. H., Aronson, D. B., and Burgess, R. R. Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J. Biol. Chem., 264: 11511-11520, 1989.

  2. Edwards, A., Darst, S., Feaver, W., Thompson, N., Burgess, R., and Kornberg, R. Purification and Lipid-layer crystallization of yeast RNA polymerase II. Proc. Natl. Acad. Sci., U.S.A., 87: 2122-2126, 1990.

  3. Thompson, N. E., Aronson, D. B., and Burgess, R. R. Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. J. Biol. Chem., 265: 7069-7077, 1990.

  4. Burgess, R. R., Thompson, N. E., Lesley, S. A., Gentry, D. R., Hager, D. A., and Brow, M. A. Use of monoclonal antibodies in studying the structure and function of E. coli RNA polymerase and sigma factors. Proceedings of the Mochida Memorial Symposium 1990, Tokyo, Japan, pp. 3-15, 1991.

  5. Buermeyer, A. B., Thompson, N. E., Strasheim, L. A., Burgess, R. R., and Farnham, P. J. The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II. Mol. Cell. Biol., 12: 2250-2259, 1992.

  6. Thompson, N. E., Hager, D. A., and Burgess, R. R. Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of E. coli RNA polymerase. Biochemistry, 31: 7003-7008, 1992.

  7. Burgess, R. R., Thompson, N. E., Hager, D. A., Strasheim, L., Aronson, D., Nolan, K., Smith C., Grabski, A. and Dickson, T. Immunoaffinity chromatography with polyol-responsive monoclonal antibodies for purification of labile proteins. Proceedings of NIGMS Symposium on Biomolecular Engineering, pp 180-184, 1994.

  8. Thompson, N. E. and Burgess, R. R. Purification of recombinant human transcription factor IIB (TFIIB) by immunoaffinity chromatography, Protein Expression Purif. 5: 469-475, 1994.

  9. Burgess, R. R. and Knuth, M. K. Purification of a recombinant protein overproduced in E. coli. In: Strategies for Protein Purification and Characterization; A Laboratory Manual, Eds: D. Marshak, J. Kadonaga, R. Burgess, M. Knuth, W. Brennan, Jr., and S.-H. Lin, Cold Spring Harbor Press, pp 1-12, 205-274, 1996.

  10. Thompson, N. E. and Burgess, R. R. Immunoaffinity purification of RNA polymerases and transcription factors using polyol-responsive monoclonal antibodies. Meth. in Enzymol. 274: 513-526, 1996.

  11. Wade, P. A., Werel, W., Fentzke, R. C., Thompson, N. E., Leykam, J. F., Burgess, R. R., Jaehning, J. A., and Burton, Z. F. A novel collection of accessory factors associated with yeast RNA polymerase II, Protein Expression Purif., 8: 85-90, 1996.

  12. Thompson, N.E. and Burgess, R. R. Immunoaffinity purification of the RAP30 subunit of human tanscription factor IIF (TFIIF). Protein Expression Purif. 17: 260-266, 1999.

ABL-MYC References

  1. Largaespada, D., Kaehler, D., Weissinger, E., Mischak, H., Mushinski, J.F., and Risser, R. (1990). The activity of an ABL-MYC retrovirus in fibroblast cells and in lymphocytes. Curr. Top. Microbiol. Immunol., 166: 91-96.,

  2. Weissinger, E. M., Mischak, H., Largaespada, D. A., Kaehler, D. A., Mitchell, T., Smith-Gill, S. J., Risser, R., and Mushinski, J. F. (1991). Induction of plasmacytomas secreting antigen-specific monoclonal antibodies with a retrovirus expressing v-abl and c-myc. Proc. Natl. Acad. Sci. USA, 88: 8735-8739.

  3. Largaespada, D. A., Kaehler, D. A., Weissinger, E., Mischak, H., Potter, M., Mushinski, J. F., and Risser, R. (1992). A retrovirus that expresses v-abl and c-myc oncogenes rapidly induces plasmacytomas. Oncogene, 7: 811-819.

  4. Weissinger, E.M., Henderson, D.W., Mischak, H., Goodnight, J., and Mushinski, J.F. (1994). Induction of plasmacytomas that secrete monoclonal anti-peptide antibodies by retroviral transformation. J. Immunol. Methods 168: 123-130
    5. .
  5. Largaespada, D. A., Jackson, M. W., Thompson, N. E., Kaehler, D. A., Byrd., L. G., and Mushinski, J.F., (1996). The ABL-MYC retrovirus generates antigen-specific plasmacytomas by in vitro infection of activated B lymphocytes from spleen and other murine lymphoid organs. J. Immunol. Methods. 197(1-2): 85-95.

  6. Huppi, K., Henderson, D., Siwarski, D. Hochman, J., Bergel, M. and G. Tuchscherer (2000). Generation of Monospecific Peptide Antibodies to the DNA Binding Domain of p53. Biotechniques 29: 1100-1106.

References Citing NeoClone Antibodies

  1. Phadtare S., and Inouye, M. (2001) Role of CspC and CspE in Regulation of Expression of RpoS and UspA, the Stress Response Proteins in Escherichia coli. J. Bacteriol. 183, 1205 - 1214.

  2. Fan, H.-Y., Merker, R. J. and Klein, H. L. (2001) High-Copy-Number Expression of Sub2p, a Member of the RNA Helicase Superfamily, Suppresses hpr1-Mediated Genomic Instability. Mol. Cell. Biol. 21, 5459 - 5470.

  3. Phadtare, S., Inouye, M., and Severinov, K. (2002) The Nucleic Acid Melting Activity of Escherichia coli CspE Is Critical for Transcription Antitermination and Cold Acclimation of Cells. J. Biol. Chem. 277, 7239 - 7245.

  4. Jishage, M., Kvint, K., Shingler, V., and Nyström, T. (2002) Regulation of factor competition by the alarmone ppGpp Genes & Dev. 16, 1260 - 1270.

  5. Thomsen, L. E., Olsen, J. E., Foster, J. W. and Ingmer, H. (2002) ClpP is involved in the stress response and degradation of misfolded proteins in Salmonella enterica serovar Typhimurium. Microbiology 148, 2727 - 2733.

  6. Luo, P. and Morrison, D.A. (2003) Transient Association of an Alternative Sigma Factor, ComX, with RNA Polymerase during the Period of Competence for Genetic Transformation in Streptococcus pneumoniae. J. Bacteriol. 185, 349 - 358.

  7. Laurie, A.D., Bernardo, L.M.D., Sze, C.C., Skärfstad, E., Szalewska-Palasz, A., Nyström, T., and Shingler, V. (2003) The Role of the Alarmone (p)ppGpp in N Competition for Core RNA Polymerase. J. Biol. Chem. 278, 1494 - 1503.

  8. Desnues, B., Cuny, C., Grégori, G., Dukan, S., Aguilaniu, H., and Nyström, T. (2003) Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells. EMBO Rep. 4, 400 - 404.

  9. Tan, Q., Prysak, M. H., and Woychik, N.A. (2003) Loss of the Rpb4/Rpb7 Subcomplex in a Mutant Form of the Rpb6 Subunit Shared by RNA Polymerases I, II, and III. Mol. Cell. Biol. 23, 3329 - 3338.

  10. Hazlett, K.R.O., Rusnak, F., Kehres, D.G., Bearden, S.W., La Vake, C. J., La Vake, M.E., Maguire, M.E., Perry, R.D., and Radolf, J.D. (2003) The Treponema pallidum tro Operon Encodes a Multiple Metal Transporter, a Zinc-dependent Transcriptional Repressor, and a Semi-autonomously Expressed Phosphoglycerate Mutase. J. Biol. Chem. 278, 20687 - 20694.

  11. Cheng, B., Zhu, C.-X., Ji, C., Ahumada, A., and Tse-Dinh, Y.-C. (2003) Direct Interaction between Escherichia coli RNA Polymerase and the Zinc Ribbon Domains of DNA Topoisomerase I. J. Biol. Chem. 278, 30705 - 30710.

  12. Mason, P.B., and Struhl, K. (2003) The FACT Complex Travels with Elongating RNA Polymerase II and Is Important for the Fidelity of Transcriptional Initiation In Vivo. Mol. Cell. Biol. 23, 8323 - 8333.

  13. Iyer, S.P.N., Akimoto, Y., and Hart, G.W. (2003) Identification and Cloning of a Novel Family of Coiled-coil Domain Proteins That Interact with O-GlcNAc Transferase. J. Biol. Chem. 278, 5399 - 5409.

  14. Krogan, N.J., Kim, M., Tong, A., Golshani, A., Cagney, G., Canadien, V., Richards, D.P., Beattie, B.K., Emili, A., Boone, C., Shilatifard, A., Buratowski, S. and Greenblatt, J. (2003) Methylation of Histone H3 by Set2 in Saccharomyces cerevisiae Is Linked to Transcriptional Elongation by RNA Polymerase II. Mol. Cell. Biol. 23, 4207 - 4218.

Epitope Tag Immunopurification

  1. Thompson, N.E., Arthur, T.M. and Burgess, R.R. (2003) Development of an epitope tag for the gentle purification of proteins by immunoaffinity chromatography: application to epitope-tagged green fluorescent protein. Analytical Biochemistry 323, 171-9.
  2. Duellman, S.J., Thompson, N.E., and Burgess, R.R. (2004)
    An epitope tag derived from human transcription factor IIB that reacts with a polyol-responsive monoclonal antibody.
    Protein Expression and Purification, 35, 147-55.

hERG antibodies

  1. Roti Roti, E.C., Myers, C.D., Ayers, R.A., Boatman, D. E., Delfosse, S.A., Chan, E.C.L., Ackerman,M.J., January, C.T., and Robertson, G.A. (2002) Interaction with GM130 during HERG ion channel trafficking. J. Biol. Chem. 277:47779-47785. PubMed Image
  2. Jones, E.M.C., Roti Roti, E., Wang, J., Delfosse, S.A., and Robertson, G.A. (2004) Cardiac IKr Channels minimally comprise hERG1a and 1b subunits. J. Biol. Chem. 279:44690-44694. Pub Med Image

© Copyright 2002-2003 Neoclone - All Rights Reserved.